The Wincom LC-100P is a specialized Semi-Preparative High-Performance Liquid Chromatography system designed not just for compound analysis, but for the actual purification and isolation of target molecules from chemical or biological mixtures. Equipped with a high-flow preparative pump, an ultra-precise UV-Vis detector, a large-volume manual injector, and a preparative scale column, the LC-100P can process and collect pure substance samples from milligram to gram scales. It is an essential tool for natural product extraction, pharmaceutical synthesis, and biotechnology purification labs.

System Configuration and Specifications

1. High-Flow Preparative Pump

• Flow Rate Range: 0.01 mL per minute to 50.00 mL per minute (or 100.00 mL per minute depending on configuration)

• Maximum Operating Pressure: 42 Megapascals (MPa) at lower flows, optimized for preparative scale pressures

• Flow Rate Accuracy: +/- 0.5 percent

• Pump Design: Dual-piston reciprocating pump with a large-capacity pump head engineered for consistent high-volume solvent delivery.

2. UV-Vis Detector (Preparative Flow Cell)

• Wavelength Range: 190 nm to 700 nm

• Wavelength Accuracy: +/- 1 nm

• Flow Cell Path Length: Optimized shorter path length flow cell to prevent signal saturation caused by high-concentration preparative samples.

• Baseline Noise: Less than or equal to 2 x 10^-5 Absorbance units

3. Preparative Injector

• Injector Type: Large-bore manual injection valve designed for high flow rates

• Loop Volume: Equipped with large-volume sample loops (standard options include 1 mL, 2 mL, or 5 mL loops)

• Construction: Heavy-duty stainless steel paths to prevent leaks during high-volume sample loading.

4. Preparative Column and Fraction Collection

• Column Type: Semi-Preparative C18 Column (typically 10 mm to 20 mm internal diameter x 250 mm length)

• Fraction Collection: Supports manual collection or seamless connection to an automated fraction collector driven by the software.

Key Features

• Large-Scale Purification Capability: Engineered specifically to transition smoothly from analytical screening to semi-preparative collection, allowing the recovery of high-purity compounds.

• Saturation-Resistant Optical Path: Features a customized optical cell that accurately reads high-concentration peaks without flattening or distorting the signal data.

• Robust Solvent Management: The pump head and fluid lines are built with chemical-resistant materials to withstand heavy organic solvent volumes over prolonged purification runs.

• Specialized Purification Software: Includes advanced chromatography software with dedicated peak-collection triggers based on time intervals or threshold absorption levels.

Primary Applications

• Natural Product Chemistry: Isolating and purifying active herbal compounds, plant extracts, and essential oils for chemical characterization.

• Synthetic Peptide and Drug Synthesis: Purifying synthetic drugs, organic compounds, and targeted chemical blocks from raw crude reaction mixtures.

• Biopharmaceutical Purification: Small-scale separation and harvesting of proteins, nucleic acids, and bio-molecules.