The Wincom LC1620A HPLC is a professional, high-pressure liquid chromatography system designed for the efficient separation, identification, and quantitative analysis of non-volatile, thermally unstable, and complex chemical compounds. This complete system integrates a high-pressure constant-flow pump, an ultra-sensitive UV-Vis detector, a precise sample injector, and a high-resolution chromatography column. Controlled by dedicated software, it delivers exceptional baseline stability and highly reproducible retention times for demanding analytical workflows.

System Configuration and Specifications

1. High-Pressure Constant-Flow Pump

• Flow Rate Range: 0.001 mL per minute to 10.000 mL per minute

• Maximum Operating Pressure: 42 Megapascals (MPa)

• Flow Rate Accuracy: +/- 0.1 percent

• Pump Design: Double-piston reciprocating parallel pump system ensuring pulsation-free solvent delivery and accurate gradient mixing.

2. UV-Vis Detector

• Wavelength Range: 190 nm to 700 nm

• Wavelength Accuracy: +/- 1 nm

• Baseline Noise: Less than or equal to 1 x 10^-5 Absorbance units

• Baseline Drift: Less than or equal to 1 x 10^-4 Absorbance units per hour

• Flow Cell Volume: 8 microliters, designed to optimize peak resolution and minimize peak broadening.

3. Manual Injector

• Injector Type: Standard Rheodyne-style 7725i manual injection valve

• Loop Volume: Standard 20 microliter sample loop installed (interchangeable for different analytical scales)

• Switch Mechanism: Smooth manual switching between load and inject positions with zero pressure spikes.

4. Chromatography Column and Oven

• Column Type: Premium C18 Reversed-Phase Analytical Column (5 micrometer particle size, 4.6 mm internal diameter x 250 mm length)

• Column Oven Temperature Range: Ambient temperature plus 5 degrees Celsius to 80 degrees Celsius

• Temperature Stability: +/- 0.1 degrees Celsius

Key Features

• Integrated Modular Architecture: The seamless communication between the pump, detector, and oven minimizes connection errors and optimizes laboratory space.

• Pulse-Free Solvent Delivery: Advanced dual-piston pump hardware eliminates pressure ripples, protecting the column and extending its operational lifespan.

• Precision Optical Layout: The detector features an easily accessible Deuterium lamp and a high-accuracy holographic grating for ultra-trace chemical identification.

• Advanced Data Workstation: Includes comprehensive chromatography software for instrument control, real-time data acquisition, automatic peak integration, and audit trail logging.

Primary Applications

• Pharmaceuticals and Drugs: Analyzing the purity, stability, active ingredients, and dissolution profiles of raw materials and finished tablets.

• Food and Beverage Testing: Quantifying nutritional additives, organic acids, vitamins, preservatives, and checking for illegal artificial colorings.

• Biochemical and Clinical Labs: Separating and analyzing amino acids, proteins, steroids, lipids, and drug metabolites in biological fluid matrices.

• Environmental Testing: Screening for hazardous organic pollutants, chemical residues, and trace micro-contaminants in municipal water and agricultural soil.