Fluorescent Quantitative PCR DetectionSystem

Real- time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, optimized qPCR regent, intuitive data analysis software, and flexible instrumentation to help harness the power of qPCR across a rich and diverse set of applications. Explore our robust solutions for your qPCR-based research.

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Category Brand:

Application:

It can be widely used for Infectious disease research, Food pathogen detection, Waterborne pathogen detection, Pharmaceutical analytics, Stem cell research, Pharmacogenomics research, Oncology and genetic disease research, Plant sciences and agricultural biotechnology.

 

Working principle:

 

The temperature step change is controlled by the semiconductor peltier to realize PCR amplification. Use high-sensitivity PD unit to detect fluorescence; Program control channel switching, non-contact excitation/detection on the top structure, coordinated with motor control x and Y axis movement to achieve 96-hole scanning. Finally, accurate analysis is carried out through powerful software.

Features:

1. Excellent temperature control performance of the instrument, Module Max heating rateheating rate 7.0℃/s.

2. No edge effect, no optical path correction, top excitation/detection, non-contact measurement.

3. Fluorescence detection adopts PD sensor with high sensitivity.

4. Long-life LED light source, stable emission wavelength, maintenance-free.

5. 4/6-channel fluorescence detection, no cross interference between channels.

6. User-friendly and fully functional software, flexible program setting, comprehensive analysis and reporting functions, all the parameters can be stored.

Technical parameters:

 

Product NameFluorescence Quantitative PCR Detection System
ModelLEIA-X4
Sample Capacity96×0.1ml PCR plate, 12×8-strip tubes, 96*0.1ml single tube (Transparent Cover)
Reaction System10~50μl
Dynamics Range1-1010 copies
Channel4
Emission LightLED
DetectorPD
Detection PathF1F2F3F4
Suitable Probe/DyeFAM/SYBR GREENVIC/JOE/ HEX/TETROX/TEXAS-REDCy5
Excitation Wavelength455~645nm
Detection Wavelength510~710nm
Fluorescence Detection RepeatabilityCV≤2%
Fluorescence Detection AccuracyCV≤3%
Fluorescence Detection Linearityr≥0.995
Module Temp. Range4-105℃(resolution:0.1℃)
Ramp Rate7.0℃/s(max)
Temp. Accuracy±0.3℃
Temp. Uniformity≤ ±0.3℃
Temp. Control ModeBlock mode
Hot-Lid Temp. Range30-110℃(resolution:0.1℃)
Scanning ModeFull plate scanning
ProgrammingMax 100 Segments for Each Program, Max 99 Cycles
Operation ModeContinuous
Scanning Time8.5s
Special FunctionAbsolute quantitative automatic analysis, relative quantification, melting curve analysis, etc.
Operation SystemMicrosoft: Windows10 x64
Power SupplyAC 100V~240V,50/60Hz
Dimension(L*W*H) Mm375*505 *375
Port MethodUSB Port
Packing Size(L*W*H) Mm645*565*605
Gross Weight48 kg

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