Antibiotic Assay Medium No.1
Antibiotic Assay Medium No.1 is used for microbiological assay of ß-lactam and other antibiotics in accordance with the United
States Pharmacopoeia.
Ingredients (Gms / Litre)
- Peptone: 6.000
- Pancreatic digest of casein: 4.000
- Yeast extract: 3.000
- Beef extract: 1.500
- Dextrose: 1.000
- Agar: 15.000
- pH after sterilization: 6.6±0.1
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.5 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by
autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Advice: Recommended for the microbiological assay of Bacitracin, Nafcillin, Cephalothin, Cephaperin, Cloxacillin,
Novobiocin, Penicillin-G
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